2HDK
Crystal Structure of Cys315Ala-Cys318Ala Mutant of Human Mitochondrial Branched Chain Aminotransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-12-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.91609 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.480, 112.187, 60.265 |
Unit cell angles | 90.00, 96.16, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.400 |
R-factor | 0.32 |
Rwork | 0.182 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 2.038 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.107 | 0.041 | 0.656 |
Number of reflections | 28489 | ||
<I/σ(I)> | 8.5 | ||
Completeness [%] | 93.2 | 84.3 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 22 to 30 % PEG1500, 100mM HEPES, 20mM DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |