2HBL
Structure of the yeast nuclear exosome component, Rrp6p, reveals an interplay between the active site and the HRDC domain; Protein in complex with Mn, Zn, and AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.043 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.846, 110.846, 79.873 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.870 - 2.300 |
| R-factor | 0.243 |
| Rwork | 0.239 |
| R-free | 0.29000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2hbj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.238 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.870 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.053 | 0.726 |
| Number of reflections | 25647 | |
| <I/σ(I)> | 11.3 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 12-14% PEG 20000, 0.1M Mes or Hepes, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
