2HAN
Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-05 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8115 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.716, 59.790, 65.179 |
Unit cell angles | 90.00, 106.70, 90.00 |
Refinement procedure
Resolution | 26.920 - 1.950 |
R-factor | 0.1816 |
Rwork | 0.180 |
R-free | 0.21706 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r0o |
RMSD bond length | 0.016 |
RMSD bond angle | 2.029 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.068 | 0.549 |
Number of reflections | 25368 | |
<I/σ(I)> | 24.2 | 3.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 21% PEG 3350, 0.1 M NaCl, 0.1 M MES, 1 mM DTT, 5 mikroM ZnCl2, 0.1 M LiCl, 10 mM MgCl2 , pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3350 | ||
10 | 1 | 2 | NaCl | ||
11 | 1 | 2 | ZnCl2 | ||
12 | 1 | 2 | LiCl | ||
13 | 1 | 2 | MgCl2 | ||
14 | 1 | 2 | H2O | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | MES | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 1 | ZnCl2 | ||
6 | 1 | 1 | LiCl | ||
7 | 1 | 1 | MgCl2 | ||
8 | 1 | 1 | H2O | ||
9 | 1 | 2 | PEG 3350 |