2HAH
The structure of FIV 12S protease in complex with TL-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 50.324, 50.324, 74.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.000 - 1.700 |
| R-factor | 0.233 |
| Rwork | 0.184 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5fiv |
| Data reduction software | MOSFLM |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.000 | |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.368 |
| Number of reflections | 12440 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 97.0 | 99 |
| Redundancy | 6.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 281.16 | 100mM Hepes, 2.5M LiCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 281.16K |
