2H4V

Crystal Structure of the Human Tyrosine Receptor Phosphatase Gamma

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97925
Spacegroup name	P 21 21 21
Unit cell lengths	74.849, 78.859, 121.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.230 - 1.550
R-factor	0.16233
Rwork	0.161
R-free	0.18130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fh7
RMSD bond length	0.013
RMSD bond angle	1.411
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.230	1.630
High resolution limit [Å]	1.550	1.550
Number of reflections	105096
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	Ammonium acetate, citrate, PEG4K, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K