2GZH
Crystal Structure of Rab11 in Complex with Rab11-Family Interacting Protein 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Detector technology | CCD |
Collection date | 2005-12-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.98175 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 64.740, 64.740, 112.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.470 |
R-factor | 0.19914 |
Rwork | 0.197 |
R-free | 0.25353 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.977 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.560 |
High resolution limit [Å] | 2.470 | 2.470 |
Rmerge | 0.067 | 0.297 |
Number of reflections | 10234 | |
<I/σ(I)> | 16.3 | 15.9 |
Completeness [%] | 99.8 | 98.6 |
Redundancy | 16 | 10 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 298 | Ammonium phosphate buffer, approximately 0.3M concentration, pH 5, VAPOR DIFFUSION, temperature 298K |