2GYY
Structure of aspartate semialdehyde dehydrogenase (ASADH) from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-10-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.479, 78.686, 243.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.100 |
| R-factor | 0.18 |
| Rwork | 0.178 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1YS4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.104 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.120 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.254 |
| Total number of observations | 28306 | |
| Number of reflections | 77734 | |
| <I/σ(I)> | 8 | 3.9 |
| Completeness [%] | 94.6 | 99.9 |
| Redundancy | 3.27 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM MES pH 6.5 50 mM calcium acetate 20% PEG 3350 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
