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2GX5

N-terminal GAF domain of transcriptional pleiotropic repressor CodY

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID23-1
Synchrotron siteESRF
BeamlineID23-1
Temperature [K]100
Detector technologyCCD
Collection date2005-12-02
DetectorADSC QUANTUM 315
Wavelength(s)0.97570
Spacegroup nameP 43 2 2
Unit cell lengths90.207, 90.207, 205.634
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution31.890 - 1.740
R-factor0.18872
Rwork0.187
R-free0.21595
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2b18
RMSD bond length0.008
RMSD bond angle1.076
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.800
High resolution limit [Å]1.7401.740
Rmerge0.0830.612
Number of reflections85808
<I/σ(I)>22.111.67
Completeness [%]98.095.2
Redundancy7.23.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP5.6291ammonium sulphate, sodium citrate, PEG 400, 20mM cGMP, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K

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