2GWR

Crystal structure of the response regulator protein mtrA from Mycobacterium Tuberculosis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.97972, 0.97952, 0.91840
Spacegroup name	P 21 21 21
Unit cell lengths	38.916, 56.598, 135.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.309 - 2.100
R-factor	0.204
Rwork	0.204
R-free	0.24400
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	1.229
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.107	0.395
Number of reflections	24268
<I/σ(I)>	11.4	3.4
Completeness [%]	99.6	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8	277	10 mM Tris, 100 mM NaCl, 1mM beta-mercaptoethanol, pH 8.0, temperature 277K
1		8	277	10 mM Tris, 100 mM NaCl, 1mM beta-mercaptoethanol, pH 8.0, temperature 277K
1		8	277	10 mM Tris, 100 mM NaCl, 1mM beta-mercaptoethanol, pH 8.0, temperature 277K