2GWD
Crystal structure of plant glutamate cysteine ligase in complex with Mg2+ and L-glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-13 |
| Detector | Large Area Detector |
| Wavelength(s) | 0.9782 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 54.810, 54.810, 518.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.460 - 2.090 |
| R-factor | 0.178 |
| Rwork | 0.175 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2GWC monomer |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.314 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.460 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 27931 | |
| <I/σ(I)> | 27.2 | 5.7 |
| Completeness [%] | 94.8 | 70.5 |
| Redundancy | 11 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 18% PEG 3350, 0.2 M magnesium acetate tetrahydrate, 0.1 M tricine, 0.05 M L-glutamate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
