2GT2
Structure of the E. coli GDP-mannose mannosyl hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-10-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 48.767, 48.767, 210.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 223.610 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.201 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rya |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 223.610 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.206 |
| Number of reflections | 34871 | |
| <I/σ(I)> | 19.4 | 4.03 |
| Completeness [%] | 92.3 | 63.8 |
| Redundancy | 4.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.4M Potassium Sodium Tartrate tetra-hydrate, pH 0.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
