2GSE
Crystal Structure of Human Dihydropyrimidinease-like 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2006-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.400, 126.100, 102.900 |
| Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
| Resolution | 29.310 - 2.400 |
| R-factor | 0.173 |
| Rwork | 0.169 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.567 |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.143 | 0.430 |
| Number of reflections | 79142 | |
| <I/σ(I)> | 9.6 | 3.36 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 18% PEG 10k, 0.1M Tris, 0.2M CaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
