2GJT

Crystal structure of the human receptor phosphatase PTPRO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95
Spacegroup name	P 65
Unit cell lengths	131.060, 131.060, 77.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.910 - 2.150
R-factor	0.18192
Rwork	0.179
R-free	0.23366
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ahs
RMSD bond length	0.017
RMSD bond angle	1.587
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.910	2.250
High resolution limit [Å]	2.150	2.150
Number of reflections	41322
Completeness [%]	99.9	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	Isopropanol, PEG4K, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 277K