2GJ6
The complex between TCR A6 and human Class I MHC HLA-A2 with the modified HTLV-1 TAX (Y5K-4-[3-Indolyl]-butyric acid) peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-20 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9829 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 223.846, 49.019, 94.170 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.560 |
| R-factor | 0.20207 |
| Rwork | 0.199 |
| R-free | 0.24988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qse |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.676 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.710 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.092 | 0.473 |
| Number of reflections | 31915 | |
| <I/σ(I)> | 15.4 | 2.2 |
| Completeness [%] | 1.0 | 93.1 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 297 | PEG8000 30%, 0.1 M Sodium Cacodylate, 0.2 M Ammonium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
