2GJ4
Structure of rabbit muscle glycogen phosphorylase in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-24 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.128, 128.128, 116.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.800 - 1.600 |
| R-factor | 0.1889 |
| Rwork | 0.187 |
| R-free | 0.22616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | In house model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.184 |
| Data reduction software | MOSFLM |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.059 | 0.466 |
| Number of reflections | 94754 | |
| <I/σ(I)> | 12 | 1.5 |
| Completeness [%] | 74.5 | 74.5 |
| Redundancy | 3.3 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 289 | 1.1mM IMP, 1.1mM Spermine, 0mM BES, 2.9mM DTT, 0.1mM EDTA, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
