2GIX
Cytoplasmic Domain Structure of Kir2.1 containing Andersen's Mutation R218Q and Rescue Mutation T309K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.674, 98.876, 98.094 |
| Unit cell angles | 90.00, 130.68, 90.00 |
Refinement procedure
| Resolution | ? - 2.020 |
| R-factor | 0.191 |
| Rwork | 0.177 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Cytoplasmic Domains of Kir2.1 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.656 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.086 | 0.347 |
| Number of reflections | 66107 | 6428 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.6 | 99.45 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 35% MPD, 0.1 NaPO4/KPO4, 50mM NaCl, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
