2GIV
Human MYST histone acetyltransferase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.305, 58.577, 119.753 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.890 - 1.940 |
| R-factor | 0.21035 |
| Rwork | 0.207 |
| R-free | 0.26198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mjb |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.122 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.890 | 2.020 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 22088 | |
| Completeness [%] | 88.6 | 41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 20% PEG3350, 0.2 M CaCl2, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
