2GHQ
CTD-specific phosphatase Scp1 in complex with peptide C-terminal domain of RNA polymerase II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.067, 78.813, 62.992 |
| Unit cell angles | 90.00, 112.17, 90.00 |
Refinement procedure
| Resolution | 48.980 - 2.050 |
| R-factor | 0.229 |
| Rwork | 0.218 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ta0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.820 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.980 | 2.140 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 35704 | |
| <I/σ(I)> | 11.94 | 2.79 |
| Completeness [%] | 95.1 | 76.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | (1)0.5M Ammonium sulfate, 0.2M Lithium sulfate, 100mM HEPES 7.5 (2) 1M sodium tartrate, 100mM Tris pH 8.5 , pH 7.5 - 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
