2GFE

Crystal structure of the GluR2 A476E S673D Ligand Binding Core Mutant at 1.54 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-13
Detector	MARRESEARCH
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	114.318, 163.301, 47.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.420 - 1.540
R-factor	0.2183
Rwork	0.212
R-free	0.22740
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1ftj
RMSD bond length	0.004
RMSD bond angle	1.154
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.600
High resolution limit [Å]	1.540	1.540
Rmerge	0.049	0.354
Number of reflections	132397
<I/σ(I)>	15	2.8
Completeness [%]	98.2	97.2
Redundancy	3.6	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	277	1 to 1 mixture of: Reservoir: 14% PEG 8K; 0.1M Na Acetate; 0.1 M Zn Acetate. Protein: 10 mg/ml 0.01 M Na HEPES pH 7.0; 0.02 M NaCl; 0.01 M Na Glutamate; 0.001 M EDTA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K