2GAF
Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2004-11-25 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.9791, 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.085, 91.260, 133.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 500.000 - 2.400 |
R-factor | 0.245 |
Rwork | 0.243 |
R-free | 0.28800 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.416 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 500.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.260 |
Number of reflections | 33681 | |
Completeness [%] | 83.4 | 68.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 20% PEG 4000, 0.24 M CaCl2, 5% glycerol, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |