2G9G
Crystal structure of His-tagged mouse PNGase C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9996 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.732, 40.747, 66.506 |
| Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.15304 |
| Rwork | 0.150 |
| R-free | 0.20711 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g2f |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.495 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.108 | 0.485 |
| Number of reflections | 13124 | |
| <I/σ(I)> | 17.8 | 3.9 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 1.6 M Li2SO4 and 100 mM Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
