2G77

Crystal Structure of Gyp1 TBC domain in complex with Rab33 GTPase bound to GDP and AlF3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-09
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979026
Spacegroup name	P 21 21 21
Unit cell lengths	72.200, 94.526, 102.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.260
R-factor	0.20726
Rwork	0.205
R-free	0.24357
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	0.987
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.260	2.260
Number of reflections	33046
<I/σ(I)>	9.9	4.93
Completeness [%]	99.0	99.9
Redundancy	5	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	10~15% polyethylene glycol 6000, 100 mM Sodium Acetate, 10% Glycerol, 20 mM NaF, 2mM AlCl3, 50 mM HEPES, pH7.0, EVAPORATION, temperature 277K
1	EVAPORATION	7	277	10~15% polyethylene glycol 6000, 100 mM Sodium Acetate, 10% Glycerol, 20 mM NaF, 2mM AlCl3, 50 mM HEPES, pH7.0, EVAPORATION, temperature 277K