2G73

Y104F mutant type 1 IPP isomerase complex with EIPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-06-30
Detector	MARRESEARCH
Wavelength(s)	1.54179
Spacegroup name	P 21 21 21
Unit cell lengths	68.935, 71.477, 91.955
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.970
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1hx3
RMSD bond length	0.029
RMSD bond angle	0.023
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.030
High resolution limit [Å]	1.970	1.970
Rmerge	0.055	0.250
Number of reflections	28124
<I/σ(I)>	6.2
Completeness [%]	99.1	92.4
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	PEG 2000 mme, MANGANESE AND MAGNESIUM CHLORIDE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K