2G50
The location of the allosteric amino acid binding site of muscle pyruvate kinase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-08-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.626, 109.014, 144.463 |
| Unit cell angles | 95.15, 93.45, 112.26 |
Refinement procedure
| Resolution | 76.470 - 1.650 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IF3W |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.151 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.074 | 0.314 |
| Number of reflections | 508417 | 33142 |
| <I/σ(I)> | 14.8 | 4.3 |
| Completeness [%] | 92.5 | 55.2 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 60 mM Succinate (pH 5.5), 5.8 mM sodium pyruvate, 2.4 mM MnCl2, 450 mM KCl, and a range of 18 to 20% PEG 8000, 150 mM Alanine, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
