2G3W
The Crystal Structure of YaeQ Protein from Xanthomonas axonopodis pv. citri
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97952, 0.97962, 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.766, 91.969, 48.031 |
| Unit cell angles | 90.00, 108.35, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 24538 | |
| <I/σ(I)> | 33.7 | 3 |
| Completeness [%] | 94.8 | 86.1 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1M Tris pH 9, 0.2M ammonium acetate, 32% PEG 4000, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
