2G1P
Structure of E. coli DNA adenine methyltransferase (DAM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.200, 71.300, 97.800 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 33.600 - 1.890 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Modified PDB entry 2DPM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 1.920 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.102 | 0.575 |
| Number of reflections | 51126 | |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.9 | 98.7 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 100 mM KCl, 10 mM MgSO4, 5-15% PEG400, 100 mM MES or HEPES (pH 6.6-7.4), pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCl | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MgSO4 | ||
| 3 | 1 | 1 | PEG400 | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | HEPES | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | KCl | ||
| 8 | 1 | 2 | MgSO4 | ||
| 9 | 1 | 2 | PEG400 |
