2G0B

The structure of FeeM, an N-acyl amino acid synthase from uncultured soil microbes

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 8-BM
Synchrotron site	APS
Beamline	8-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-16
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9422, 0.9793
Spacegroup name	I 41 2 2
Unit cell lengths	182.832, 182.832, 287.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.750 - 3.000
Rwork	0.255
R-free	0.29200
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	0.917
Data reduction software	MOSFLM
Data scaling software	SCALA (CCP4_3.1.20)
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.100	3.150
High resolution limit [Å]	3.000	3.000
Rmerge	0.081	0.160
Total number of observations		110846
Number of reflections	48895
<I/σ(I)>	7.8737	1.31
Completeness [%]	100.0	100
Redundancy	16.4	16.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	289	4.06 M NaCl, 50 mM glycine, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K