2FZE

Crystal structure of the binary complex of human glutathione-dependent formaldehyde dehydrogenase with ADP-ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	113
Wavelength(s)	1.2820
Spacegroup name	P 43 21 2
Unit cell lengths	79.234, 79.234, 310.964
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 1.900
Rwork	0.183
R-free	0.20900
Structure solution method	SAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.098	0.434
Number of reflections	79380	7762
<I/σ(I)>	14.8
Completeness [%]	100.0	100
Redundancy	27.2	23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.1	277	15% PEG 8000, 100 mM potassium phosphate, 100 uM zinc sulphate, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K