2FUG
Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97564 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 135.080, 266.112, 201.731 |
Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.300 |
R-factor | 0.265 |
Rwork | 0.265 |
R-free | 0.29800 |
Structure solution method | MIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.630 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.988 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.158 | 0.552 |
Total number of observations | 84269 | |
Number of reflections | 195669 | 26742 |
<I/σ(I)> | 6.1 | 1.7 |
Completeness [%] | 92.7 | 89.2 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K |