2FP4

Crystal structure of pig GTP-specific succinyl-CoA synthetase in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-16
Detector	ADSC QUANTUM 4
Wavelength(s)	1.11588
Spacegroup name	P 65
Unit cell lengths	132.610, 132.610, 72.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	116.038 - 2.080
R-factor	0.214
Rwork	0.195
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.900
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	116.038	72.990	2.210
High resolution limit [Å]	2.080	6.640	2.100
Rmerge	0.055	0.019	0.362
Total number of observations		8067	22254
Number of reflections	43486	1442	6210
<I/σ(I)>	11.9	28.6	2
Completeness [%]	99.3
Redundancy	5.5	5.6	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	5 mM GTP, 10 mM MgCl2, 20% w/v polylethylene glycol 3350, 150 mM KF, VAPOR DIFFUSION, HANGING DROP, temperature 294K