2FO5
Crystal structure of recombinant barley cysteine endoprotease B isoform 2 (EP-B2) in complex with leupeptin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2005-10-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.115869 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.370, 51.730, 115.655 |
| Unit cell angles | 86.32, 86.46, 79.63 |
Refinement procedure
| Resolution | 15.000 - 2.200 |
| Rwork | 0.201 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.059 | 0.030 | 0.284 |
| Number of reflections | 48898 | 5319 | 3231 |
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 89.9 | ||
| Redundancy | 3.3 | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.4 M LiSO4 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
