2FO1
Crystal Structure of the CSL-Notch-Mastermind ternary complex bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-07-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.093, 96.785, 243.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.510 - 3.120 |
| R-factor | 0.273 |
| Rwork | 0.273 |
| R-free | 0.34000 |
| Structure solution method | SIRAS, MIRAS, MR |
| Starting model (for MR) | 1TTU PDB ENTRY 1OT8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.230 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Number of reflections | 47404 | |
| <I/σ(I)> | 25 | 3.2 |
| Completeness [%] | 98.3 | 88.2 |
| Redundancy | 13.5 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 277 | 10% PEG 10K, 0.15M Ammonium Acetate, 0.1M BisTris, 10% ethylene glycol, pH 5.5, MICROBATCH, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 10% PEG 10K | ||
| 2 | 1 | 1 | 0.15M Ammonium Acetate | ||
| 3 | 1 | 1 | 0.1M BisTris | ||
| 4 | 1 | 1 | 10% ethylene glycol | ||
| 5 | 1 | 2 | 10% PEG 10K | ||
| 6 | 1 | 2 | 0.15M Ammonium Acetate | ||
| 7 | 1 | 2 | 10% ethylene glycol |
