2FMX
An open conformation of switch I revealed by Sar1-GDP crystal structure at low Mg(2+)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-11 |
Detector | ENRAF-NONIUS GX-21 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.825, 61.993, 71.126 |
Unit cell angles | 90.00, 107.58, 90.00 |
Refinement procedure
Resolution | 39.070 - 1.820 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.070 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.091 | 0.411 |
Number of reflections | 35092 | |
<I/σ(I)> | 11.8 | 2 |
Completeness [%] | 88.6 | 63.8 |
Redundancy | 3.1 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.6 | 298 | 28%(w/v)Polyethylene Glycol-4000, 100mM sodium acetate, 0.2M ammonium sulfate, pH 5.6, EVAPORATION, temperature 298K |