2FMA

Structure of the Alzheimer's Amyloid Precursor Protein (APP) Copper Binding Domain in 'small unit cell' form, atomic resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	31.289, 32.488, 50.088
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.850 - 0.850
R-factor	0.1305
Rwork	0.131
R-free	0.14970
Structure solution method	Rigid body refinement
Starting model (for MR)	2fjz
RMSD bond length	0.026
RMSD bond angle	0.036
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	0.870
High resolution limit [Å]	0.850	0.850
Number of reflections	41418
<I/σ(I)>		1.7
Completeness [%]	90.5	43.8
Redundancy		1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1M HEPES pH 8.0, 28-32% (w/v) PEG 10000, VAPOR DIFFUSION, HANGING DROP, temperature 295K