2FLU
Crystal Structure of the Kelch-Neh2 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Wavelength(s) | 1.23983 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.757, 92.069, 46.314 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.500 |
| R-factor | 0.18048 |
| Rwork | 0.179 |
| R-free | 0.19867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u6d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.211 |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.400 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.077 | 0.456 |
| Number of reflections | 50551 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 94.7 | 64.6 |
| Redundancy | 6.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | 100 mM TrisHCl 200 mM MgCl2 30% PEG 4000, pH 8.5, VAPOR DIFFUSION, temperature 277K |
