2FLP
Binary complex of the catalytic core of human DNA polymerase iota with DNA (template G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.367, 98.367, 202.497 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.510 - 2.400 |
| R-factor | 0.2328 |
| Rwork | 0.228 |
| R-free | 0.27616 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE ALZ |
| RMSD bond length | 0.060 |
| RMSD bond angle | 3.182 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.055 |
| Number of reflections | 22540 |
| <I/σ(I)> | 16.5 |
| Completeness [%] | 98.3 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | pH 6.5, VAPOR DIFFUSION, HANGING DROP |
