2FJU

Activated Rac1 bound to its effector phospholipase C beta 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-04
Detector	MARRESEARCH
Wavelength(s)	1.009
Spacegroup name	P 32 2 1
Unit cell lengths	185.822, 185.822, 93.823
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.200
Rwork	0.208
R-free	0.22610
Structure solution method	MIR, MAD, MR
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	93710

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	8% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K