2FJM
The structure of phosphotyrosine phosphatase 1B in complex with compound 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.431, 86.621, 139.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.100 |
| R-factor | 0.19284 |
| Rwork | 0.192 |
| R-free | 0.21676 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1pty |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.254 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | CNX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 61477 | |
| <I/σ(I)> | 12.3 | 1.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.83 | 7.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 284 | PEG 3350, MGCL2, HEPES, pH 7.00, VAPOR DIFFUSION, SITTING DROP, temperature 284K |
