2FJ9

High resolution crystal structure of the unliganded human ACBP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-02
Detector	MARRESEARCH
Wavelength(s)	0.91
Spacegroup name	P 65 2 2
Unit cell lengths	43.824, 43.824, 177.247
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.830 - 1.600
R-factor	0.196
Rwork	0.196
R-free	0.22900
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	2.200
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.620
High resolution limit [Å]	1.560	1.560
Number of reflections	14797
<I/σ(I)>	31.9
Completeness [%]	95.9	98.5
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	25% PEG-MME 550, 100mM MES, pH 6.5, 10mM ZnSO4, 3% 1-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K