2FH7
Crystal structure of the phosphatase domains of human PTP SIGMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 61 |
| Unit cell lengths | 94.333, 94.333, 123.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.670 - 2.000 |
| R-factor | 0.19199 |
| Rwork | 0.190 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lar |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.148 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.760 | 1.910 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.093 | 0.011 |
| Number of reflections | 55608 | |
| <I/σ(I)> | 6 | 0.6 |
| Completeness [%] | 99.0 | 93.3 |
| Redundancy | 9.3 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | Protein(10mg/ml) was crystallized in (15% PEG3350,100mM Succinic Acid)., pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
