2FGE
Crystal structure of presequence protease PreP from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 110.834, 114.332, 162.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.660 - 2.100 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.25600 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.311 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.660 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.119 | 0.417 |
| Number of reflections | 116872 | |
| <I/σ(I)> | 10.4 | 2.26 |
| Completeness [%] | 96.5 | 86.6 |
| Redundancy | 4.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22% PEG 6000, 0.1M Hepes, 0.025M magnesium chloride, 0.1% beta-mercaptoethanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
