2FFV
Human ppGalNAcT-2 complexed with manganese and UDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2005-07-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97472 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 153.228, 153.228, 110.144 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.100 - 2.750 |
R-factor | 0.2251 |
Rwork | 0.225 |
R-free | 0.28600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Separate catalytic domains of PDB entry 1XHB in which nonconserved residues were changed to alanine |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.100 |
High resolution limit [Å] | 2.750 |
Number of reflections | 34265 |
Completeness [%] | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | PEG, Hepes, MnCl2, EDTA, mercaptoethanol, UDP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |