2FAH
The structure of mitochondrial PEPCK, Complex with Mn and GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-03-20 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 |
Unit cell lengths | 74.595, 90.852, 103.339 |
Unit cell angles | 64.23, 73.74, 71.18 |
Refinement procedure
Resolution | 32.810 - 2.090 |
R-factor | 0.1891 |
Rwork | 0.187 |
R-free | 0.23366 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2FAG |
RMSD bond length | 0.010 |
RMSD bond angle | 1.305 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.180 |
High resolution limit [Å] | 2.090 | 4.520 | 2.090 |
Rmerge | 0.106 | 0.045 | 0.581 |
Number of reflections | 127447 | ||
Completeness [%] | 95.3 | 98.8 | 91.9 |
Redundancy | 3.3 | 3.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 16% PEG6000, 0.1M HEPES, n-octanoylsucrose, GDP, MnCl2, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |