2F9J
3.0 angstrom resolution structure of a Y22M mutant of the spliceosomal protein p14 bound to a region of SF3b155
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-21 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 41 |
| Unit cell lengths | 71.271, 71.271, 102.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.250 - 3.000 |
| R-factor | 0.22128 |
| Rwork | 0.219 |
| R-free | 0.26586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 2f9d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.328 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.059 | 0.023 | 0.460 |
| Number of reflections | 10288 | ||
| Completeness [%] | 99.8 | 98.2 | 100 |
| Redundancy | 4.2 | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 60% NaH2PO4/40% K2HPO4 0.5M beta-alanine, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
