2F8Z
Crystal structure of human FPPS in complex with zoledronate and isopentenyl diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2004-01-29 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00033 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 112.155, 112.155, 65.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.710 - 2.600 |
| R-factor | 0.226 |
| Rwork | 0.220 |
| R-free | 0.28000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2f8c |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNX (2002) |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.072 | 0.481 |
| Number of reflections | 13248 | |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 292 | 1.2M sodium potassium phosphate, 25% glycerol, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
