2F7T

Crystal structure of the catalytic domain of Mos1 mariner transposase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-24
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97624
Spacegroup name	P 41 21 2
Unit cell lengths	44.243, 44.243, 206.047
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.250
R-factor	0.19345
Rwork	0.189
R-free	0.27685
Structure solution method	MAD
RMSD bond length	0.025
RMSD bond angle	2.017
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.350
High resolution limit [Å]	2.250	2.250
Rmerge	0.130
Number of reflections	10257
<I/σ(I)>	13	6.3
Completeness [%]	97.1	85.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	24-26% PEG 4000, 0.1M Tris, 0.005M MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	24-26% PEG 4000, 0.1M Tris, 0.005M MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K