2F69
Ternary complex of SET7/9 bound to AdoHcy and a TAF10 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-08-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.380, 83.380, 95.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.140 - 1.300 |
| R-factor | 0.15008 |
| Rwork | 0.149 |
| R-free | 0.17412 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o9s |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.038 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.140 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.060 | 0.505 |
| Number of reflections | 94528 | |
| <I/σ(I)> | 17 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.26 | 9.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.89-2.04 M (NH4)2SO4, 0.1 M Bis TRIS pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
