2F49
Crystal structure of Fus3 in complex with a Ste5 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.11590 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.622, 95.210, 101.662 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b9f |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 69388 | |
| <I/σ(I)> | 15.3 | 2.4 |
| Completeness [%] | 95.3 | 83.2 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.1 | 293 | 18% PEG3350, 0.1M Mes, 10% MPD, 0.2M KSCN, pH 6.1, VAPOR DIFFUSION, temperature 293K |
