2F49
Crystal structure of Fus3 in complex with a Ste5 peptide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000749 | biological_process | response to pheromone triggering conjugation with cellular fusion |
| A | 0000750 | biological_process | pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| A | 0001403 | biological_process | invasive growth in response to glucose limitation |
| A | 0004672 | molecular_function | protein kinase activity |
| A | 0004674 | molecular_function | protein serine/threonine kinase activity |
| A | 0004705 | molecular_function | JUN kinase activity |
| A | 0004707 | molecular_function | MAP kinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0006468 | biological_process | protein phosphorylation |
| A | 0007254 | biological_process | JNK cascade |
| A | 0010494 | cellular_component | cytoplasmic stress granule |
| A | 0010526 | biological_process | retrotransposon silencing |
| A | 0035556 | biological_process | intracellular signal transduction |
| A | 0042597 | cellular_component | periplasmic space |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0043332 | cellular_component | mating projection tip |
| A | 0043409 | biological_process | negative regulation of MAPK cascade |
| A | 0046827 | biological_process | positive regulation of protein export from nucleus |
| A | 0051301 | biological_process | cell division |
| A | 0071507 | biological_process | pheromone response MAPK cascade |
| A | 0106310 | molecular_function | protein serine kinase activity |
| B | 0000749 | biological_process | response to pheromone triggering conjugation with cellular fusion |
| B | 0000750 | biological_process | pheromone-dependent signal transduction involved in conjugation with cellular fusion |
| B | 0001403 | biological_process | invasive growth in response to glucose limitation |
| B | 0004672 | molecular_function | protein kinase activity |
| B | 0004674 | molecular_function | protein serine/threonine kinase activity |
| B | 0004705 | molecular_function | JUN kinase activity |
| B | 0004707 | molecular_function | MAP kinase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0006468 | biological_process | protein phosphorylation |
| B | 0007254 | biological_process | JNK cascade |
| B | 0010494 | cellular_component | cytoplasmic stress granule |
| B | 0010526 | biological_process | retrotransposon silencing |
| B | 0035556 | biological_process | intracellular signal transduction |
| B | 0042597 | cellular_component | periplasmic space |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0043332 | cellular_component | mating projection tip |
| B | 0043409 | biological_process | negative regulation of MAPK cascade |
| B | 0046827 | biological_process | positive regulation of protein export from nucleus |
| B | 0051301 | biological_process | cell division |
| B | 0071507 | biological_process | pheromone response MAPK cascade |
| B | 0106310 | molecular_function | protein serine kinase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG A 601 |
| Chain | Residue |
| A | TYR24 |
| A | GLY25 |
| A | GLU45 |
| A | LYS49 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MG B 602 |
| Chain | Residue |
| B | TYR24 |
| B | GLY25 |
| B | GLU45 |
| B | LYS49 |
| site_id | AC3 |
| Number of Residues | 2 |
| Details | BINDING SITE FOR RESIDUE SCN A 501 |
| Chain | Residue |
| A | ILE44 |
| A | LYS42 |
| site_id | AC4 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SCN B 503 |
| Chain | Residue |
| A | LYS49 |
| A | PRO50 |
| A | LEU51 |
| A | PHE52 |
| B | LYS49 |
| B | PRO50 |
| B | LEU51 |
| B | PHE52 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SCN B 504 |
| Chain | Residue |
| A | ALA337 |
| B | ARG189 |
| B | VAL193 |
| B | LYS199 |
| site_id | AC6 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE SCN A 505 |
| Chain | Residue |
| A | ARG189 |
| A | VAL193 |
| A | MET194 |
| A | LYS199 |
| B | GLU336 |
| B | ALA337 |
| site_id | AC7 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE SCN A 506 |
| Chain | Residue |
| A | THR185 |
| A | ARG186 |
| A | TRP187 |
| B | THR339 |
| site_id | AC8 |
| Number of Residues | 8 |
| Details | BINDING SITE FOR RESIDUE SCN B 507 |
| Chain | Residue |
| A | LYS341 |
| B | THR185 |
| B | ARG186 |
| B | TRP187 |
| B | GLY225 |
| B | HOH624 |
| B | HOH666 |
| B | HOH702 |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 25 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGEGAYGVVCsAthkptgeiv.........AIKK |
| Chain | Residue | Details |
| A | LEU19-LYS43 |
| site_id | PS00108 |
| Number of Residues | 13 |
| Details | PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ViHrDLKpsNLLI |
| Chain | Residue | Details |
| A | VAL133-ILE145 |
| site_id | PS01351 |
| Number of Residues | 103 |
| Details | MAPK MAP kinase signature. FdkplfalrtlREikilkhfkheniitifniqrpdsfenfnevyiiqelmqtdlhrvistqmlsddhiqyfiyqtlravkvlhgsnvih..........RDlKpsnllinsnC |
| Chain | Residue | Details |
| A | PHE47-CYS149 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027 |
| Chain | Residue | Details |
| A | ASP137 | |
| B | ASP137 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159 |
| Chain | Residue | Details |
| A | LEU19 | |
| A | LYS42 | |
| B | LEU19 | |
| B | LYS42 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphothreonine => ECO:0000269|PubMed:1628831 |
| Chain | Residue | Details |
| A | VAL180 | |
| B | VAL180 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:1628831 |
| Chain | Residue | Details |
| A | PHE182 | |
| B | PHE182 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047 |
| Chain | Residue | Details |
| A | LYS345 | |
| B | LYS345 |
Catalytic Information from CSA
| site_id | CSA1 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| A | SER141 | |
| A | ASP137 |
| site_id | CSA2 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| B | SER141 | |
| B | ASP137 |
| site_id | CSA3 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| A | LYS139 | |
| A | ASP137 |
| site_id | CSA4 |
| Number of Residues | 2 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| B | LYS139 | |
| B | ASP137 |
| site_id | CSA5 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| A | THR185 | |
| A | LYS139 | |
| A | ASP137 |
| site_id | CSA6 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| B | THR185 | |
| B | LYS139 | |
| B | ASP137 |
| site_id | CSA7 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| A | ASN142 | |
| A | LYS139 | |
| A | ASP137 |
| site_id | CSA8 |
| Number of Residues | 3 |
| Details | Annotated By Reference To The Literature 1ir3 |
| Chain | Residue | Details |
| B | ASN142 | |
| B | LYS139 | |
| B | ASP137 |
