2F02
Crystal Structure of LacC from Enterococcus Faecalis in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-28 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | .9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.463, 62.739, 93.454 |
| Unit cell angles | 90.00, 99.91, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.20308 |
| Rwork | 0.201 |
| R-free | 0.23996 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0016) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 | 0.281 |
| Number of reflections | 57959 | |
| <I/σ(I)> | 11.2 | 2.37 |
| Completeness [%] | 98.0 | 96.2 |
| Redundancy | 3.2 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.4M NACITRATE, 0.1M TRIS PH 8.5, 0.01M ATP, CRYO-XYLITOL, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
